Is it possible to limit TL0 to posting in one category?

I don’t think that is possible without a plugin. The requirements for TL1 are all based on reading

activity default setting
Read posts 30 tl1 requires read posts
Visit topics 5 tl1 requires topics entered
Total reading time 10 tl1 requires time spent mins

Maybe you want to keep the “see” permission for “everyone”, otherwise the category cannot be seen by visitors and crawlers.

You can use Administrative Bulk Operations to change the permissions of many categories at the same time

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